Virus Separation and Purification Methods

Virus Separation and Purification Methods

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This reference presents many contributions made by Dr Alfred Polson during his 41 years of research into the physicochemical properties of plant and animal viruses - detailing his timesaving approaches to the characterization, extraction, separation, concentration and purification of viruses, proteins, antibodies and biopolymers.;Describing successful laboratory techniques featuring the Beckman preparative centrifuge, this reference: examines the electro-extraction process for isolating the maximum amount of viruses from infected plant material; analyzes zone electrophoresis in sugar concentration gradients as a powerful tool for purifying entero-, insect born and insect viruses; contains material on the use of a reorienting gradient centrifuge rotor to separate components of the haemolymph of the mollusc Turbo sarmaticus as a model for virus separation; explains the construction of a modified thin-layer ultracentrifuge rotor for concentrating viruses into suspension rather than pellets; elucidates the use of inserts in ultracentrifuge tubes in order to decrease the time of centrifuging; and introduces a method for determining pure viruse suspension densities.;This resource is intended for microbiologists, virologists, biochemists, molecular and cell biologists, immunologists, biochemical and bioprocess engineers, chemical engineers and graduate students in these disciplines.2a and 2b are recorded the photometric diagrams of the precipitin bands of the material recovered from the top and bottom compartments ... 3 the diagram obtained with Type I virus against homologous guinea-pig immune serum is given.

Title:Virus Separation and Purification Methods
Author: Alfred Polson
Publisher:CRC Press - 1993-07-29

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